Zetasizer Nano ZSP
If you do not have an active NUCORE account, contact the ANTEC Director.
What to Bring for Your Analysis
- Disposable plastic cuvettes for size measurements
- Disposable folded capillary cells for zeta potential measurements
ANTEC does not provide disposables or small equipment (pipettes) for your experimental needs.
The Zetasizer Nano ZSP from Malvern Panalytical is used for measurement of protein size, electrophoretic mobility, zeta potential of nanoparticles and can optionally be used for microrheology of protein and polymer solutions. It is equipped with integrated heating/cooling and allows for a chamber temperature range from 2 to 90° C.
Applications and Detection Limits
The Zetasizer Nano ZSP allows users to measure molecular weight, concentration and zeta potential of particles ranging in size from 0.3 to 10,000 nm.
Technique limitations are movements which are not Brownian, such as particle aggregation, sedimentation, and multiple scattering. DLS assumes that the particles are spherical; however you can still measure non-spherical particles.
Nanoparticle Tracking Analysis (NTA, Nanosight300 available at ANTEC) is complimentary to Dynamic Light Scattering (DLS, Zetasizer Nano ZSP) as it allows the determination of relative proportion of smaller particles in the sample. DLS measures size distribution and polydispersity of the sample; however, large particles in the sample have higher scattering light intensity and mask small particles; therefore, size distribution should be verified with NTA which can detect particle populations exhibiting a 20 nm difference.
|Particle size and molecular size:|
|Measurement range:||0.3 nm – 10.0 microns* (diameter)|
|Measurement principle:||Dynamic Light Scattering|
|Minimum sample volume:||12 µL|
|Accuracy:||Better than +/-2% on NIST traceable latex standards|
|Precision / Repeatability:||Better than +/-2% on NIST traceable latex standards|
|Sensitivity:||0.1 mg/mL (Lysozyme)|
|Measurement range:||3.8nm – 100 microns (diameter)*|
|Measurement principle:||Electrophoretic Light Scattering|
|Minimum sample volume:||150µL (20µL using diffusion barrier method)|
|Accuracy:||0.12µm.cm/V.s for aqueous systems using NIST SRM1980 standard reference material|
Please acknowledge the use of ANTEC equipment when data obtained in our core is published, used for proposal submissions, or included in your presentations! Acknowledgement helps demonstrate ANTEC’s value to the Northwestern University Research community and contributes to our efforts to secure funding for new instruments and services. See sample text.