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SQI: Simpson Querry Institute

CFX Connect

Reservations

If you do not have an active NUCORE account, contact the ANTEC Director.

RESERVE

What to Bring for Your Analysis

ANTEC does not provide disposables or small equipment (pipettes) for your experimental needs. 

Equipment Specifications

Description

The CFX-Connect real-time PCR detection system from Bio-Rad offers two-target analysis for SYBR® Green/EvaGreen and duplex experiments in a 96-well format. The high average temperature ramp rate allows for a short run time. The solid-state optical technology individually illuminates and detects fluorescence from each well with high sensitivity and no cross talk.

At ANTEC, users can spin down PCR plates before performing the analysis on the CFX-Connect.

Applications

Amplification, PCR, qPCR, Real-Time PCR, Genomics, Cloning, Cycle Sequencing, Gene Expression studies.

Technical Specifications

This table contains technical information about the CFX Connect and what kind of analyses it can perform.
Maximum ramp rate, °C/sec 5
Average ramp rate, °C/sec 3.3
Heating and cooling method Peltier
Lid, °C Heats up to 105
Temperature
Range, °C 0–100
Accuracy, °C ±0.2 of programmed target at 90°C
Uniformity, °C ±0.4 well-to-well within 10 sec of arrival at 90°C
Gradient
Operational range, °C 30–100
Programmable span, °C 1–24
Optical Detection
Excitation 3 filtered LEDs
Detection 3 filtered photodiodes
Range of excitation/emission wavelengths, nm 450–580
Sensitivity Detects 1 copy of target sequence in human genomic DNA
Dynamic range 10 orders of magnitude
Scan Time
All channels, sec 12
FAM/SYBR® Green only, sec 3

 

Acknowledging ANTEC

Please acknowledge the use of ANTEC equipment when data obtained in our core is published, used for proposal submissions, or included in your presentations! Acknowledgement helps demonstrate ANTEC’s value to the Northwestern University Research community and contributes to our efforts to secure funding for new instruments and services.  See sample text.