CFX Connect
Reservations
If you do not have an active NUCORE account, contact the ANTEC Director.
What to Bring for Your Analysis
- 96 well low profile pcr plate
- Micropipette and tips
ANTEC does not provide disposables or small equipment (pipettes) for your experimental needs.
Equipment Specifications
Description
The CFX-Connect real-time PCR detection system from Bio-Rad offers two-target analysis for SYBR® Green/EvaGreen and duplex experiments in a 96-well format. The high average temperature ramp rate allows for a short run time. The solid-state optical technology individually illuminates and detects fluorescence from each well with high sensitivity and no cross talk.
At ANTEC, users can spin down PCR plates before performing the analysis on the CFX-Connect.
Applications
Amplification, PCR, qPCR, Real-Time PCR, Genomics, Cloning, Cycle Sequencing, Gene Expression studies.
Technical Specifications
| Maximum ramp rate, °C/sec | 5 |
| Average ramp rate, °C/sec | 3.3 |
| Heating and cooling method | Peltier |
| Lid, °C | Heats up to 105 |
| Temperature | |
| Range, °C | 0–100 |
| Accuracy, °C | ±0.2 of programmed target at 90°C |
| Uniformity, °C | ±0.4 well-to-well within 10 sec of arrival at 90°C |
| Gradient | |
| Operational range, °C | 30–100 |
| Programmable span, °C | 1–24 |
| Optical Detection | |
| Excitation | 3 filtered LEDs |
| Detection | 3 filtered photodiodes |
| Range of excitation/emission wavelengths, nm | 450–580 |
| Sensitivity | Detects 1 copy of target sequence in human genomic DNA |
| Dynamic range | 10 orders of magnitude |
| Scan Time | |
| All channels, sec | 12 |
| FAM/SYBR® Green only, sec | 3 |
Acknowledging ANTEC
Please acknowledge the use of ANTEC equipment when data obtained in our core is published, used for proposal submissions, or included in your presentations! Acknowledgement helps demonstrate ANTEC’s value to the Northwestern University Research community and contributes to our efforts to secure funding for new instruments and services. See sample text.