If you do not have an active NUCORE account, contact the ANTEC Director.
What to Bring for Your Analysis
- A sample concentration should be between 1x108 – 2x109 particles/mL (that range roughly corresponds to 10–100 particles in a field of view). It is recommended to produce a 1 mL batch of nanoparticles at a material concentration of 10 mg/ml, and dilute the initial batch 1:100 or 1:1000. A sample volume of 1.5 – 2.0 mL should be provided, as it takes sample volume of ~0.6 – 0.7 mL to reach the flow cell.
- 1 mL syringes – preferably latex and silicone free (latex and silicone can introduce more bubbles) – use Norm-Ject syringes
- Clean solvent (aqueous solutions only) and DI water (dust particulates in solvent/water can show up in the Nanosight and skew your measurements)
The NanoSight300 from Malvern Instruments utilizes the properties of light scattering and Brownian motion to obtain the size distribution and concentration measurement of particles in liquid suspension. Nanoparticle Tracking Analysis technology combines laser light scattering microscopy with a CCD camera and enables visualization and recording of nanoparticles in solution. The Nanosight is equipped with 488 nm and 642 nm interchangeable laser modules and allows for fluorescent particles analysis.
Applications and Detection Limits
The Nanosight allows for rapid (3-5 min per run) size distribution and concentration analysis of nanoparticles from 10 nm to 2,000 nm.
- Only aqueous solvents and non-hazardous materials can be tested on the ANTEC Nanosight.
- The software tracks many particles individually and calculates their hydrodynamic diameters. NTA assumes a spherical particle and ascribes a diameter to that particle. NTA is not accurate for measuring particles with asymmetry exceeding an aspect ratio of 3.
- At a lower detection limit around 10 nm, NTA can be applied for measuring hard, perfectly spherical nanoparticles with a high refractive index.
- At an upper detection limit around 1–2 microns, particles do not move enough to get robust size measurements.
Please acknowledge the use of ANTEC equipment when the data obtained at our core is published, used for submission of grants and proposals, or for poster presentations! Acknowledgement helps to demonstrate ANTEC’s value to the Northwestern University Research community and contributes to our efforts to secure funding for new instruments and services. See sample text.